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C3306

Sigma-Aldrich

Cloruro di calcio

≥99.0%

Sinonimo/i:

CaCl2.2H2O, Calcium chloride, Calcium dichloride

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100 G
27,30 €
250 G
52,40 €
500 G
94,00 €

27,30 €

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100 G
27,30 €
250 G
52,40 €
500 G
94,00 €

About This Item

Formula empirica (notazione di Hill):
CaCl2 · 2H2O
Numero CAS:
10035-04-8
Peso molecolare:
147.01
Numero CE:
233-140-8
Numero MDL:
MFCD00149613
Codice UNSPSC:
12352302
ID PubChem:
24892603
NACRES:
NA.31

27,30 €

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Grado

Molecular Biology

Livello qualitativo

200

Saggio

≥99.0%

Stato

powder

Punto di fusione

176 °C (dec.) (lit.)

Solubilità

H2O: 294 mg/mL, clear, colorless

Attività estranea

DNase, RNase, protease, none detected

Stringa SMILE

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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Categorie correlate

Applicazioni

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Azioni biochim/fisiol

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.[1]

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H319

Classi di pericolo

Eye Irrit. 2

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
0000435482
0000435481
0000435480
0000435480
0000435481

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Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Hui Sun Leong et al.
Nature communications, 5, 3947-3947 (2014-05-24)
Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in
Bradley T Estes et al.
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The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well
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Scientific reports, 10(1), 4399-4399 (2020-03-12)
The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of
Denisa Hathazi et al.
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Reversible infantile respiratory chain deficiency (RIRCD) is a rare mitochondrial myopathy leading to severe metabolic disturbances in infants, which recover spontaneously after 6-months of age. RIRCD is associated with the homoplasmic m.14674T>C mitochondrial DNA mutation; however, only ~ 1/100 carriers develop
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Domande

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

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