Osteoblast Growth Medium has been optimized for the in vitro cultivation of human osteoblasts. The fetal calf serum in the medium is strictly pretested for optimal performance. The medium is optimized for primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Recommended for:Human Osteoblasts (HOB)
Componenti
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial
Qualità
All lots of PromoCell Osteoblast Growth Medium are subjected to comprehensive quality control tests using primary human osteoblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested osteoblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Nota sulla preparazione
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / ml
Stoccaggio e stabilità
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.
Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.
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