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A3563

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep

affinity isolated antibody, buffered aqueous glycerol solution

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204,00 €
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353,00 €

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0.5 ML
204,00 €
1 ML
353,00 €

About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

204,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Origine biologica

sheep

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous glycerol solution

Reattività contro le specie

mouse

tecniche

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

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Categorie correlate

Descrizione generale

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Sheep anti-mouse IgG (whole molecule) F(ab′)2 fragment is specific for the F(ab′)2 of mouse IgG. The antibody preparation consists only of the F(ab′)2 fragment of sheep IgG as determined by SDS-PAGE. No contamination with sheep IgG whole molecule is observed.

Immunogeno

Mouse IgG

Applicazioni

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000), western blot (1:30,000), and immunohistochemistry (at 1:50 dilution in formalin-fixed, paraffin-embedded sections). The antibody may also be used for dot blot and immunoblot assays using a working dilution of 1:30,000.
ELISAs using alkaline phosphatase conjugated sheep anti-mouse IgG whole molecule antibody were performed to measure the antibody response in the sera of mice immunixed to T. crassiceps. 1:1000 at 37 degrees for one hour and developed using p-nitrophenyl (Sigma).

Stato fisico

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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