76216
Papain from Carica papaya
solution, light brown, ≥10 U/mg protein (~25 mg/ml)
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50 MG
151,00 €
250 MG
227,00 €
1 G
751,00 €
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Cambia visualizzazione
50 MG
151,00 €
250 MG
227,00 €
1 G
751,00 €
About This Item
Prodotti consigliati
Origine biologica
papaya
Livello qualitativo
Stato
solution
Attività specifica
≥10 U/mg protein (~25 mg/ml)
PM
Mr ~23000
tecniche
cell culture | mammalian: suitable
single cell analysis: suitable
Colore
light brown
Temperatura di conservazione
2-8°C
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Descrizione generale
Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.[1]
Applicazioni
Papain from Carica papaya has been used:
Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.
Azioni biochim/fisiol
Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food[1], baking, and dairy industries.[5]
Definizione di unità
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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