70215

2,3-Naphthalenedicarboxaldehyde

suitable for fluorescence

• Sinonimo/i: NDA
• Formula condensata: C₁₀H₆(CHO)₂
• Numero CAS: 7149-49-7
• Peso molecolare: 184.19
• Beilstein: 2207267
• Numero MDL: MFCD00016618
• Codice UNSPSC: 12352108
• ID PubChem: 329762349
• NACRES: NA.32

Proprietà

• Stato: crystals
• Impurezze: ≤2% mono- and dicarboxylic acid (¹H-NMR)
• Punto di fusione: 131-133 °C (lit.); 132-135 °C
• Fluorescenza: λ_ex 420 nm; λ_em ~480 nm in 0.1 M borate pH 9.3 (after derivatization with glycine [~90 μM glycine, ~20 μM N^-])
• Compatibilità: suitable for fluorescence
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: [H]C(=O)c1cc2ccccc2cc1C([H])=O
• InChI: 1S/C12H8O2/c13-7-11-5-9-3-1-2-4-10(9)6-12(11)8-14/h1-8H; ZIPLKLQPLOWLTM-UHFFFAOYSA-N

Descrizione

Descrizione generale

2,3-Naphthalenedicarboxaldehyde, also known as NDA, is a novel fluorescent derivatization agent. The fluorescence max. excitation of NDA is in the range of 424 nm and max. emission is achieved at 484nm.[1]

2,3-Naphthalenedicarboxaldehyde is a fluorescent derivatization agent of primary amines, amino acids, and small peptides. The reaction between the amino compounds and NDA results in highly fluorescent and stable derivative compounds.

Applicazioni

2,3-Naphthalenedicarboxaldehyde is used as a fluorescent labelling agent for quantification of Histamine in human immunoglobulin preparations.[1] NAD is used as a derivatization agent for glutathione to quantify protein glutathionylation.[2] It is a suitable reagent used for the development of fluorescence-based microtiter plate assay for γ-glutamylcyclotransferase.[3]

Caratteristiche e vantaggi

NDA based fluorescent assays have the following benefits over O-Phthalaldehyde (OPA):

• High Reaction rate.
• High Fluorescence quantum yield.
• No uncommon excitation wavelength.
• No side reactions.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H315,H319,H335
• Consigli di prudenza: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves