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67060

Sigma-Aldrich

Methyl Green

zinc chloride salt, for microscopy (Bact., Bot., Hist.)

Sinonimo/i:

Ethyl Green

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About This Item

Formula condensata:
C27H35BrClN3 · ZnCl2
Numero CAS:
7114-03-6
Peso molecolare:
653.24
Colour Index:
42590
Beilstein:
3914106
Numero CE:
230-415-4
Numero MDL:
MFCD00151094
Codice UNSPSC:
12171500
ID PubChem:
329760277
NACRES:
NA.47

Grado

for microscopy (Bact., Bot., Hist.)

Forma fisica

powder

pH

0.2-1.8, yellow to blue

Punto di fusione

>300 °C (lit.)

Solubilità

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥900 at 624-634 nm in water

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
RRKSQWOQZFIQKW-UHFFFAOYSA-J

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Applicazioni

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Azioni biochim/fisiol

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Risultati analitici

Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts.

Avvertenze

Danger

Indicazioni di pericolo

H314,H335,H411

Classi di pericolo

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

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Sigma-Aldrich

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K R Fox et al.
European journal of histochemistry : EJH, 36(3), 263-270 (1992-01-01)
Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At
Evaluation of apoptosis as a mechanism of follicular cell atresia in the ovaries of cattle (bos indicus) and buffalo (bubalus bubalis) fetuses.
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Monocyte chemoattractant protein-1 in human atheromatous plaques.
Nelken NA, et al.
The Journal of Clinical Investigation, 88, 1121-1121 (1991)
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Osteoarthritis (OA) is a highly prevalent, disabling joint disease with no existing therapies to slow or halt its progression. Cartilage degeneration hallmarks OA pathogenesis, and pannexin 3 (Panx3), a member of a novel family of channel proteins, is upregulated during
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