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Sigma-Aldrich

Atto 532

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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1 MG
291,00 €

291,00 €


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1 MG
291,00 €

About This Item

Numero MDL:
Codice UNSPSC:
12352108
NACRES:
NA.25

291,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Richiedi un ordine bulk

Nome Commerciale

BioReagent

Saggio

≥90% (HPCE)

Produttore/marchio commerciale

ATTO-TEC GmbH

Fluorescenza

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Descrizione generale

Atto 532 is a new label with high molecular absorption (115,000) and quantum yield (0.90) as well as sufficient Stoke′s shift between excitation and emission maximum. It is optimized for excitation with frequency doubled Nd:YAG-Laser, and is characterized by high photostability.

Applicazioni

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 532 has been conjugated with the secondary antibodies for STED (stimulated emission depletion) microscopy and immunofluorescence studies.[1][2]

Note legali

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
STED microscopy reveals nanoparticle assemblies
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Toshiro Saito et al.
Nanotechnology, 22(44), 445708-445708 (2011-10-13)
We fabricated platinum bowtie nanostructure arrays producing fluorescence enhancement and evaluated their performance using two-photon photoluminescence and single-molecule fluorescence measurements. A comprehensive selection of suitable materials was explored by electromagnetic simulation and Pt was chosen as the plasmonic material for
Chunlai Chen et al.
Nucleic acids research, 35(9), 2875-2884 (2007-04-14)
Hybridization of nucleic acids with secondary structure is involved in many biological processes and technological applications. To gain more insight into its mechanism, we have investigated the kinetics of DNA hybridization/denaturation via fluorescence resonance energy transfer (FRET) on perfectly matched

Articoli

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Domande

Recensioni

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