UFC5030
Filtri centrifughi Amicon® Ultra, MWCO 30 kDa
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa
Sinonimo/i:
Concentratore centrifugo, MWCO di 30 kDa, volume del campione 0,5 mL
About This Item
Prodotti consigliati
Materiali
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Livello qualitativo
Sterilità
non-sterile
Nome Commerciale
Amicon®
Produttore/marchio commerciale
Amicon® Ultra
Parametri
0.5 mL sample volume
tecniche
protein extraction: suitable
protein purification: suitable
Lunghezza
4.99 cm (1.96 in.)
Diametro
10.8 mm
Superficie filtrante
1 cm2
Volume
0.5 mL
Dimensione pori
30 kDa MWCO
Condizioni di spedizione
ambient
Descrizione generale
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
® ®
Applicazioni
- ®
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Caratteristiche e vantaggi
- ®
- μμ
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: da 10 a 30C.
- Reverse spin capability provides consistent recoveries
Linkage
Stato fisico
Note legali
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Codice della classe di stoccaggio
10-13 - German Storage Class 10 to 13
Certificati d'analisi (COA)
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Articoli
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Metodi di filtrazione e selezione di filtri a membrana per la purificazione e produzione di nanoparticelle
Protocolli
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Contenuto correlato
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
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