It is recommended to choose a molecular weight cut-off that is at least two times smaller than the molecular weight of the target molecules.
UFC5010
Filtri centrifughi Amicon® Ultra, MWCO 10 kDa
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
1 recensori su 2 hanno ricevuto un prodotto campione o hanno partecipato a una promozione
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa
Sinonimo/i:
Concentratore centrifugo, MWCO di 10 kDa, volume del campione 0,5 mL
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
8 PKG
66,50 €
24 PKG
161,00 €
96 PKG
507,00 €
500 PKG
2.190,00 €
Scegli un formato
Cambia visualizzazione
8 PKG
66,50 €
24 PKG
161,00 €
96 PKG
507,00 €
500 PKG
2.190,00 €
About This Item
Codice UNSPSC:
41104916
eCl@ss:
36100101
NACRES:
NB.24
Prodotti consigliati
Materiali
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Livello qualitativo
Sterilità
non-sterile
Nome Commerciale
Amicon®
Produttore/marchio commerciale
Amicon® Ultra
Parametri
0.5 mL sample volume
tecniche
protein extraction: suitable
protein purification: suitable
Lunghezza
4.99 cm (1.96 in.)
Diametro
10.8 mm
Superficie filtrante
1 cm2
Volume
0.5 mL
Dimensione pori
10 kDa MWCO
Condizioni di spedizione
ambient
Descrizione generale
® >
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
® ®
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
® ®
Applicazioni
- ®
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Caratteristiche e vantaggi
- ®
- μμ
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: da 10 a 30C.
- Reverse spin capability provides consistent recoveries
Stato fisico
Codice colore: trasparente
Note legali
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Articoli
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Metodi di filtrazione e selezione di filtri a membrana per la purificazione e produzione di nanoparticelle
Protocolli
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Contenuto correlato
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
-
What is the tolerance for molecular weight with these spin columns? Could you reasonably purify a protein of 15 kDa using these columns, or are the tolerances too high and would result in loss of protein into the filtrate?
1 risposta-
Utile?
-
-
Is this filter suitable for purifying MHC-bound peptides as filtrate/eluate while trapping proteins and antibodies as retentate following Immunoprecipitation?
1 risposta-
This product is not suitable for separating protein/peptides of different sizes.
Utile?
-
-
If a centrifuge is unable to achieve a spinning speed of 14000 X g as recommended in the protocol, is it possible to compensate by spinning the samples for a longer duration?
1 risposta-
Yes, it is possible to compensate for a lower centrifuge spinning speed by increasing the duration. This can be achieved by calculating the K factor, which determines the adjustment needed in time.
Utile?
-
-
se puede utilizar los UFC501024 para concentrar una proteina glicosilada?
1 risposta-
Yes, Amicon devices like UFC501024 can be used to concentrate any protein greater than about 20 kDa.
Utile?
-
-
Dear Sir/Mam, can you let me know how many tubes is 8 pkg? Is it 8 tubes? Best regards, Alejandra
1 risposta-
It is 8 tubes per pack.
Utile?
-
-
What is the maximum RPM that this can be spun to ensure the membrane does not perforate?
1 risposta-
The recommended G force for the Amicon® Ultra-0.5 filter is 14,000 g & 1,000 g reverse spin when using a Fixed-Angle (35 º) Rotor.
Please refer to the brochure for more information: details: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf
Utile?
-
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
