MABS2157

Anti-CH25H Antibody, clone 18G9

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 18G9, monoclonal
• PM: calculated mol wt 31.75 kDa; observed mol wt ~34 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: human, mouse
• Confezionamento: antibody small pack of 100 μg
• tecniche: ELISA: suitable; western blot: suitable
• Isotipo: IgG1κ
• Sequenza dell’epitopo: Unknown
• N° accesso ID proteina: NP_003947
• N° accesso UniProt: O95992
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CH25H(9023)

Descrizione

Descrizione generale

Cholesterol 25-hydroxylase (UniProt: O95992; also known as EC:1.14.99.38, Cholesterol 25-monooxygenase, h25OH) is encoded by the CH25H gene (Gene ID: 9023) in human. CH25H is a multi-pass membrane glycoprotein with three transmembrane domains (aa 38-58; 84-104; and 121-141) that catalyzes the formation of 25-hydroxycholesterol from cholesterol, which in turn leads to repression of cholesterol biosynthesis. It is also reported to play a role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H is an interferon inducible enzyme that has broad-spectrum antiviral functions. Stimulation of macrophage Toll-like receptors (TLR) is also reported to increase its expression and synthesis of 25-hydroxycholesterol. Treatment of naïve B cells with nanomolar concentrations of 25-hydroxycholesterol is shown to suppress IL-2-mediated stimulation of B cell proliferation, repress activation induced cytidine deaminase expression, and reduce IgA production. Deletion of CH25H gene in mouse is shown to increase in serum IgA. In testis tissue, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. (Ref.: Bauman, DR., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(39); 16764-16769).

Specificità

Clone 18G9 is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylae (CH25H).

Immunogeno

His-tagged full length recombinant human Cholesterol 25-hydroxylase.

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cholesterol 25-hydroxylase (CH25H) in lysate from HEK293T cells overexpressing cholesterol 25-hydroxylase (CH25H).

Tested Applicaitons

Western Blotting Analysis: A representative lot detected CH25H in Western Blotting applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9; Tuong, Z.K., et. al. (2016). PLoS One. 11(1):e0147179).

ELISA Analysis: A representative lot detected CH25H in ELISA applications (Bauman, D.R., et al. (2009). Proc Natl Acad Sci USA. 106(39):16764-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CH25H, clone 18G9, Cat. No. MABS2157, is a mouse monoclonal antibody that detects Cholesterol 25-hydroxylase (CH25H) and is tested for use in ELISA and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Stoccaggio e stabilità

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable