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MABN2719

Sigma-Aldrich

Anti-Neurofilament M/NEFM Antibody, clone 2H3

Sinonimo/i:

160 kDa neurofilament protein, NF-M, Neurofilament 3, Neurofilament medium polypeptide, Neurofilament triplet M protein

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

2H3, monoclonal

PM

calculated mol wt 96 kDa
observed mol wt ~160 kDa

Purificato mediante

using protein G

Reattività contro le specie

rat, human, mouse

Confezionamento

antibody small pack of 100

tecniche

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

Sequenza dell’epitopo

Unknown

N° accesso ID proteina

N° accesso UniProt

Temperatura di conservazione

2-8°C

Informazioni sul gene

rat ... Nefm(24588)

Specificità

Clone 2H3 is a mouse monoclonal antibody that detects Neurofilament-M (NEFM)

Immunogeno

Membrane preparations from E14-E15 rat brain tissue.

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue extracts.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Neurofilament medium polypeptide (NF-M) in Rat brain tissue extract.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Neurofilament medium polypeptide (NF-M) in mouse brain tissue extract.

Immunohistochemistry Applications: A representative lot detected Neurofilament medium polypeptide (NF-M) in Immunohistochemistry applications (Clugston, R.D., et al. (2010). Am J Respir Cell Mol Biol. 42(3):276-85; Lysakowski, A., et al. (2011). J Neurosci. 31(27):10101-14; Kridsada, K., et al. (2018). Cell Rep. 23(10):2928-2941; Latremoliere, A., et al. (2018). Cell Rep. 24(7):1865-1879.e.9).

Immunocytochemistry Analysis: A representative lot detected Neurofilament medium polypeptide (NF-M) in Immunocytochemistry applications (Latremoliere, A., et al. (2018). Cell Rep. 24(7):1865-1879.e.9).

Immunofluorescence Analysis: A representative lot detected Neurofilament medium polypeptide (NF-M) in Immunofluorescence applications (Latremoliere, A., et al. (2018). Cell Rep. 24(7):1865-1879.e.9).

Western Blotting Analysis: A representative lot detected Neurofilament medium polypeptide (NF-M) in Western Blotting applications (Fernandez-Cerado, C., et al. (2021). J Neural Transm (Vienna). 128(4):575-587).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

Neurofilament medium polypeptide (UniProt: P12839; also known as NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein) is encoded by the Nefm (also known as Nef3, Nfm) gene (Gene ID: 24588) in rat. Neurofilaments are intermediate filaments that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. They are built from three intertwined protofibrils, which themselves are composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neurofilaments contain a number of repeats of the tripeptide KSP (Lys-Ser-Pro) and NF-M is phosphorylated on a number of the serine residues in this motif by protein kinase A and C. Phosphorylation of NF-M results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Deletion of the phosphorylated tail domain of NF-M is reported to inhibit radial growth of axons and reduce their conduction velocities. However, mice expressing NF-M subunits that lack phosphorylation at KSP sites along the tail domain have normal axon calibers and conduction velocities, indicating that the NF-M tail domain, but not tail phosphorylation is crucial for axon radial growth. The level of phosphorylation is shown to be altered developmentally and coincides with a change in the neurofilament function. Mutations in the rod domain region of NF-M have been identified in early onset Parkinson s disease. Also, in the brains of Alzheimer s disease patients, a reciprocal relationship between O-GlcNAcylation and phosphorylation of NF-M has been reported where reduced O-GlcNAcylation is accompanied with increased KSP phosphorylation in NF-M. (Ref.: Yuan, A., et al. (2017). Cold Spring Harb. Perspect. Biol. 9(4); a018309; Yuan, A., et al. (2012). J. Cell Sci. 125(14); 3257-3263).

Stato fisico

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Ricostituzione

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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