MAB5448

Anti-Neurofilament NF-H Antibody, clone TA51

culture supernatant, clone TA51, Chemicon^®

• Sinonimo/i: NF-H
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: culture supernatant
• Tipo di anticorpo: primary antibodies
• Clone: TA51, monoclonal
• Reattività contro le specie: human, rat, hamster, monkey
• Produttore/marchio commerciale: Chemicon^®
• tecniche: ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NM_021076.2
• N° accesso UniProt: P12036
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NEFH(4744) ; rat ... Nefh(24587) ; rhesus monkey ... Nefh(717705)

Descrizione

Specificità

Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}.

Immunogeno

Epitope: carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH

Applicazioni

Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g

for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}

Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.

Optimal working dilutions must be determined by end user.

Stato fisico

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable