MAB1407P
Anti-Macrophages/Granulocytes Antibody, clone OX-41
clone OX-41, Chemicon®, from mouse
Sinonimo/i:
CD172 antigen-like family member A, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, SHP substrate 1, SHP substrate-1, Signal-regulatory protein alpha-1, signal-regulatory protein alpha
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
OX-41, monoclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
mouse, human, rat
Produttore/marchio commerciale
Chemicon®
tecniche
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotipo
IgG2a
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... SIRPA(140885)
Categorie correlate
Descrizione generale
Macrophages and granulocytes express Signal regulatory protein (SIRP), which is a transmembrane glycoprotein involved in the down regulation of tyrosine kinase receptor signal transduction induced by cell adhesion, growth factors or insulin. They are immunoglobulin-like cell surface receptor for CD47. By CD47 binding there is a negative regulation of phagocytosis, mast cell activation and dendritic cell activation that prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Specificità
Expected to cross-react with rat and mouse.
MAB1407P recognizes rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons. This monoclonal binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a and CD47.
Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.
Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.
Immunogeno
Rat peritoneal macrophages.
Applicazioni
Immunohistochemistry:
Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).
Western Blotting:
A previous lot of this antibody was used in western blot.
Flow cytometry:
1:100-1:200 of a previous lot was used. (Use 10 μL of working dilution for 10^6 cells in 100 μL).
Optimal working dilutions must be determined by the end user.
Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).
Western Blotting:
A previous lot of this antibody was used in western blot.
Flow cytometry:
1:100-1:200 of a previous lot was used. (Use 10 μL of working dilution for 10^6 cells in 100 μL).
Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
This Anti-Macrophages/Granulocytes Antibody, clone OX-41 is validated for use in FC, IC, IH, IH(P), WB for the detection of Macrophages/Granulocytes.
Qualità
Routinely evaluated by Immunocytochemistry on Macrophages/Granulocytes.
Immunocytochemistry Analysis:
Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.
Immunocytochemistry Analysis:
Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.
Descrizione del bersaglio
56 kDa
Linkage
Replaces: MAB1407
Stato fisico
ImmunoAffinity Purified
Purified mouse monoclonal IgG2a in buffer containing 0.09% sodium azide.
Stoccaggio e stabilità
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Risultati analitici
Control
Macrophages, monocytes, lymphoid organs.
Macrophages, monocytes, lymphoid organs.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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