AB8345
Anti-MMP-14 Antibody
serum, Chemicon®
Sinonimo/i:
MT1-MMP
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
human
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... MMP14(4323)
Categorie correlate
Specificità
The antibody recognizes native and recombinant proteins.
Immunogeno
Catalytic domain of MT1-MMP
Applicazioni
Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
Immunocytochemistry: 5-10 μg / ml
Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.
FACS Analysis: 5 μg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.
Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.
FACS Analysis: 5 μg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Stato fisico
Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.
Stoccaggio e stabilità
Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.
Risultati analitici
Control
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Albert G Remacle et al.
Oncotarget, 8(2), 2781-2799 (2016-11-12)
The invasion-promoting MT1-MMP is a cell surface-associated collagenase with a plethora of critical cellular functions. There is a consensus that MT1-MMP is a key protease in aberrant pericellular proteolysis in migrating cancer cells and, accordingly, a promising drug target. Because
David Alonso-Escolano et al.
British journal of pharmacology, 141(2), 241-252 (2003-12-24)
1. Matrix metalloproteinase-2 (MMP-2) plays a role in agonist- and tumour cell-induced platelet aggregation (TCIPA). 2. MMP-2 is synthesized as a proenzyme and is activated at the cell surface by membrane type-1 matrix metalloproteinase (MT1-MMP, MMP-14). 3. The significance of
Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells.
Cavallo-Medved, D; Mai, J; Dosescu, J; Sameni, M; Sloane, BF
Journal of Cell Science null
Albert G Remacle et al.
The Journal of biological chemistry, 288(28), 20568-20580 (2013-06-05)
Proteolytic activity of cell surface-associated MT1-matrix metalloproteinase (MMP) (MMP-14) is directly related to cell migration, invasion, and metastasis. MT1-MMP is regulated as a proteinase by activation and conversion of the latent proenzyme into the active enzyme, and also via inhibition
Mengying Xing et al.
iScience, 27(2), 108840-108840 (2024-02-02)
N-α-acetyltransferase D (NatD) mediates N-α-terminal acetylation of histone H4 (Nt-Ac-H4), but its role in breast cancer metastasis remains unknown. Here, we show that depletion of NatD directly represses the expression of FOXA2, and is accompanied by a significant reduction in
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