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Merck
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Documenti fondamentali

616455

Sigma-Aldrich

TGF-β1, Human, Recombinant, CHO Cell Line

Sinonimo/i:

rhTGF-β1, Transforming Growth Factor-β1

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About This Item

Codice UNSPSC:
12352202

Saggio

≥97% (SDS-PAGE)

Livello qualitativo

Stato

lyophilized

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze

Impurezze

≤1 EU/μg Endotoxin (EU/μg TGF-β1)

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Descrizione generale

Recombinant, human transforming growth factor-β1 expressed in CHO cells. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigeminal neurinomal cells. Induces the accumulation of extracellular matrix in various diseases.

Azioni biochim/fisiol

ED₅₀ = 0.04-0.1 ng/ml

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from a sterile filtered solution in 50 µg BSA/µg TGF-β1, 35% acetonitrile, 0.1% TFA.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
Reconstitute to a concentration ≥1 µg/ml in 4 mM sterile HCl containing ≥0.1% HSA or BSA.

Altre note

Shibuya, H., et al. 1996. Science272, 1179.
Bottlinger, E.P., et al. 1995. Proc. Natl. Acad. Sci. USA93, 5877.
Lomo, J., et al. 1995. J. Immunol.154, 1634.
Marushige, K., and Marushige, Y. 1995. Anticancer Res.14, 2419.
Yamazaki, M., et al. 1994. Am. J. Pathol. 144, 221.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3


Certificati d'analisi (COA)

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Holly B Hindman et al.
Investigative ophthalmology & visual science, 51(4), 1935-1942 (2009-11-13)
Purpose. To characterize phenotypic differences between anterior and posterior corneal keratocytes after stimulation with the profibrotic agent transforming growth factor-beta1 (TGF-beta1) in vitro. Methods. Sixteen corneas from healthy felines were obtained immediately after death. Lamellar dissection was performed to separate
Hon Wai Koon et al.
The American journal of pathology, 177(5), 2300-2309 (2010-10-05)
Substance P (SP) and the neurokinin-1 receptor (NK-1R) are involved in the development of colitis and mucosal healing after colonic inflammation. We studied whether SP modulates colonic fibrosis by using a chronic model of trinitrobenzenesulfonic acid (TNBS)-induced colitis in wild-type
Nagat Frara et al.
Connective tissue research, 59(1), 30-44 (2017-04-13)
Transforming growth factor beta 1 (TGFbeta-1) and connective tissue growth factor (CCN2) are important mediators of tissue repair and fibrosis, with CCN2 functioning as a downstream mediator of TGFβ-1. Substance P (SP) is also linked to collagen production in tenocytes.
Jeeranan Manokawinchoke et al.
Cell death & disease, 10(10), 761-761 (2019-10-09)
Mechanical force regulates periodontal ligament cell (PDL) behavior. However, different force types lead to distinct PDL responses. Here, we report that pretreatment with an intermittent compressive force (ICF), but not a continuous compressive force (CCF), promoted human PDL (hPDL) osteogenic
M Herranz-Itúrbide et al.
Redox biology, 40, 101841-101841 (2021-01-26)
Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions

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