574597-M

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

liquid, Calbiochem^®

• Sinonimo/i: Anti-SOD
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: sheep
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: mouse, rat, human, monkey
• Produttore/marchio commerciale: Calbiochem^®
• Disponibilità: not available in Canada,
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.

Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.

Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O₂-) to molecular oxygen (O₂) and hydrogen peroxide (H₂O₂).

This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

Immunogeno

Human

highly purified, human erythrocyte SOD Cu/Zn.

Applicazioni

Immunoblotting (1:1000)

Immunoelectrophoresis (see comments)

Frozen sections (see comments)

Paraffin sections (see comments)

Free-floating sections (see comments)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In glycine-buffered saline, pH 7.4.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
Human red blood cell lysate

Altre note

Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.

This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable