218701

Casein Kinase II, Human, Recombinant, E. coli

Casein Kinase II, Human, Recombinant, E. coli Recombinant, is expressed in E. coli. A cyclic nucleotide-independent Ser/Thr protein kinase composed of catalytic α-subunits and regulatory β-subunits.

• Sinonimo/i: rhCKII
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in E. coli
• Livello qualitativo: 100
• Stato: liquid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Research area: CELL SIGNALING

Note: 1 KU = 1000 units.

Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits and regulatory β-subunits in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.

Applicazioni

Casein kinase II (CK2) has been used:

• has been used as a marker for CK2 subunits, to analyze CK2 expression in human retinal extract. [1]
• to investigate the phosphorylation of the C-terminal domain of uroplakin IIIa and assess its potential regulation by CK2.[2]
• to investigate the phosphorylation events and their impact on spectrin tetramer formation and neuronal cell function.[3]

Azioni biochim/fisiol

Casein kinase II (CK2) is an active serine/threonine protein kinase that plays a pivotal role in cell growth and division. It is responsible for phosphorylating various nuclear proteins involved in DNA metabolism, transcription factors, and oncogenes, thus influencing their activity. [4] It also regulates osteogenesis, adipogenesis, chondrogenesis, neuron differentiation, and immune response pathways. [5] CK2 plays a crucial role in activating the Janus Kinase/signal transducer and activator of transcription (JAK/STAT) pathway, which contributes to cell survival, differentiation, and proliferation. Furthermore, CK2 is involved in activating the nuclear factor kappa B (NF-κB) pathway. [5] It is highly expressed in different hematological malignancies, including acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), and chronic myeloid leukemia (CML). [6]

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.

Stato fisico

In 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON^™ X-100 Detergent, pH 7.5.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Yamaguchi, Y., et al. 1998. Nucleic Acids Res.26, 3854.
Chester, N., and Marshak, D.R. 1993. Anal. Biochem.209, 284.
Russo, G.L., et al. 1992. J. Biol. Chem.267, 20317.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable