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Sigma-Aldrich

Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate

clone 4G10®, Upstate®, from mouse

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

4G10®, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Upstate®

tecniche

immunoprecipitation (IP): suitable

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

phosphorylation (pTyr)

Informazioni sul gene

human ... PID1(55022)

Descrizione generale

Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Specificità

Recognizes tyrosine-phosphorylated proteins from all species.

Applicazioni

Anti-Phosphotyrosine Antibody, recombinant clone 4G10, agarose conjugate detects level of Phosphotyrosine & has been published & validated for use in IP.
Research Category
Signaling
Research Sub Category
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.

Caratteristiche e vantaggi

Format: Gel Immobilized

Qualità

Routinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

Immunoprecipitation Analysis:
10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.

Descrizione del bersaglio

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Stato fisico

Gel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension.
Protein A purified

Stoccaggio e stabilità

Stable for 6 months at 2-8ºC from date of receipt.

Risultati analitici

Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Xiquan Liang et al.
Journal of the American Society for Mass Spectrometry, 18(11), 1932-1944 (2007-09-18)
Protein phosphorylation regulates many aspects of cellular function, including cell proliferation, migration, and signal transduction. An efficient strategy to isolate phosphopeptides from a pool of unphosphorylated peptides is essential to global characterization using mass spectrometry. We describe an approach employing

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