1.11662

CHAPS

for biochemistry

• Sinonimo/i: CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio] propane sulfonate), 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Formula empirica (notazione di Hill): C₃₂H₅₈N₂O₇S
• Numero CAS: 75621-03-3
• Peso molecolare: 614.88
• Numero MDL: MFCD00012116
• Codice UNSPSC: 12352107
• NACRES: NA.05

Proprietà

• product name: CHAPS (3-[(3-Cholamidopropyl)-dimethylammonio]-propane- sulfonate), for biochemistry
• Livello qualitativo: 100
• Forma fisica: solid
• PM: micellar avg mol wt 6150
• Numero d'aggregazione: 10
• pH: 6 (100 g/L in H₂O)
• CMC: 6 mM (20-25°C)
• Punto di fusione: 157 °C
• Temp. transizione: cloud point >100 °C
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1; UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descrizione

Applicazioni

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Risultati analitici

Assay (ex N): ≥ 99 %
Identity (IR-spectrum): passes test
Absorbance A (280 nm; 1 %; 1 cm; water): ≤ 0.1
Absorbance A (260 nm; 1 %; 1 cm; water): ≤ 0.1

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302
• Consigli di prudenza: P264 - P270 - P301 + P312 - P501
• Classi di pericolo: Acute Tox. 4 Oral
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable