07-303

Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody

Upstate^®, from rabbit

• Sinonimo/i: acetyl-CoA carboxylase 1, acetyl-CoA carboxylase-alpha, acetyl-Coenzyme A carboxylase alpha
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: canine, human
• Reattività contro le specie (prevista in base all’omologia): bovine (immunogen homology), chicken (immunogen homology), rat (immunogen homology), mouse (immunogen homology), dog (immunogen homology)
• Confezionamento: antibody small pack of 25 μg
• Produttore/marchio commerciale: Upstate^®
• tecniche: western blot: suitable
• N° accesso NCBI: NP_942131
• N° accesso UniProt: Q13085
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: phosphorylation (pSer79)
• Informazioni sul gene: human ... ACACA(31)

Descrizione

Descrizione generale

Acetyl CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by the reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones, glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors of the cell, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl CoA carboxylase is thus enhanced. Acetyl CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Specificità

Recognizes Acetyl CoA Carboxylase phosphorylated at Serine 79, MW 257 kDa.

Immunogeno

Epitope: N-terminal

KLH conjugated synthetic peptide (C-HM RSSM[pS]GLHLVK) corresponding to amino acid 73-85 of rat Acetyl CoA Carboxylase.

Applicazioni

Anti-phospho-Acetyl CoA Carboxylase (Ser79) Antibody is an antibody against phospho-Acetyl CoA Carboxylase (Ser79) for use in WB.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Qualità

Evaluated by Western Blot on Lambda phosphatase-treated and untreated A375 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected endogenous phospho-Acetyl CoA Carboxylase (Ser79) in lysate from Lambda phosphatase-treated and untreated A375 cells.

Descrizione del bersaglio

257 kDa

Linkage

Replaces: 04-1009

Stato fisico

Format: Purified

Protein A chromatography

Protein A purified in buffer containing in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% NaN3.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.