05-1530-M

Anti-Importin beta-1 Antibody, clone 3H14

clone 3H14, from mouse

• Sinonimo/i: Karyopherin subunit beta-1, Nuclear factor P97, importin 1, importin 90, importin beta-1 subunit, karyopherin (importin) beta 1, karyopherin beta 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 3H14, monoclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_002256.2
• N° accesso UniProt: Q14974
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... KPNB1(3837)

Descrizione

Descrizione generale

Importin beta-1 (also known as Importin subunit beta-1) facilitates nuclear protein import. This occurs either though association with a nuclear localization signal (NLS) and an adapter protein , such as an importin-alpha subunit, which which binds to nuclear, or directly where the protein acts as autonomous nuclear transport receptor.
Import of macromolecules containing a NLS requires a heterodimer of importin alpha and beta subunits also called as karyopherins. Importin alpha binds the cytoplasmic NLS-containing protein and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.

Specificità

This antibody recognizes Importin beta-1.

Immunogeno

Epitope: Unknown

Recombinant protein corresponding to human Importin beta-1.

Applicazioni

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Importin beta-1 Antibody, clone 3H14 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Importin beta-1 also known as Karyopherin subunit beta-1, Nuclear factor P97.

Qualità

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Importin beta-1 on 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~ 97 kDa

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable