MABF1968

Anti-Microglobulin beta2 Antibody, clone BBM.1

clone BBM.1, from mouse

• Szinonimák: B2M
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• klón: BBM.1, monoclonal
• application: FACS; IHC; WB; inhibition assay
• faj reaktivitás: human
• technika/technikák: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); inhibition assay: suitable; western blot: suitable

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: BBM.1, monoclonal
• faj reaktivitás: human
• kiszerelés: antibody small pack of 25 μg
• technika/technikák: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); inhibition assay: suitable; western blot: suitable
• izotípus: IgG2bκ
• NCBI elérési szám: NP_004039
• UniProt elérési szám: P61769
• kiszállítva: ambient
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... B2M(567)

Leírás

Általános leírás

Beta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). Beta-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum beta-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin′s lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency.

Egyediség

Clone BBM.1 detects Beta-2-microglobulin in human.

Immunogén

MOLT-4, a Human acute lymphoblastic leukemia cell line.

Alkalmazás

Anti-Microglobulin beta2, clone BBM.1, Cat. No. MABF1968, is a mouse monoclonal antibody that detects Microglobulin beta2 and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assays, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Microglobulin beta2 in Immunohistochemistry applications (Josson, S., et. al. (2011). Cancer Res. 71(7):2600-10).

Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).

Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).

Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.

Research Category
Inflammation & Immunology

Minőség

Evaluated by Western Blotting in MOLT-4 cell lysates

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.

Cél megnevezése

~14 kDa observed; 13.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Fizikai forma

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable