ABE446

Anti-Monomethyl Histone H1 (Lys26) Antibody

from rabbit, purified by affinity chromatography

• Szinonimák: Histone H1.3, Histone H1c, Histone H1s-2
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: polyclonal
• application: DB; IP; WB
• faj reaktivitás: human
• technika/technikák: dot blot: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 1

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: human
• technika/technikák: dot blot: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI elérési szám: NP_005311
• UniProt elérési szám: P16402
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: monomethylation (Lys26)
• Géninformáció: human ... HIST1H1D(3007)

Leírás

Általános leírás

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes, and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

Egyediség

This antibody recognizes the N-terminus of Monomethyl Histone H1 (Lys26) methylated at Lys26.

Immunogén

KLH-conjugated linear peptide corresponding to the N-terminus of human Monomethyl Histone H1 (Lys26).

Alkalmazás

Detect Histone H1 using this rabbit polyclonal antibody, Anti-Monomethyl Histone H1 (Lys26) Antibody validated for use in western blotting, Dot Blot & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Monomethyl Histone H1 (Lys26) in 500 µg of HeLa acid extract.
Dot Blot Specificity Analysis: This antibody detected Monomethyl Histone H1 (Lys26) in in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Minőség

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monomethyl Histone H1 (Lys26) in 10 µg of HeLa acid extract.

Cél megnevezése

~32 kDa observed

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable