17-10520
Magna NuCLEAR™ RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41105331
NACRES:
NA.84
Javasolt termékek
Minőségi szint
Általános leírás
Magna Nuclear RIP Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq).
Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Cross-linked can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. In contrast native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. For less well understood proteins and protein complexes often both approaches are used.
Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Cross-linked can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. In contrast native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. For less well understood proteins and protein complexes often both approaches are used.
Alkalmazás
Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.
Kiszerelés
Kit capacity: 24 RNA-binding protein immunoprecipitation assays using a cross-linked nuclear lysate
Komponensek
10X Glycine;10X PBS;Nuclei Isolation Buffer;RIP Cross-Linked Lysis Buffer;Protein A/G Magnetic Beads;Nuclear RIP Dilution Buffer;Low Salt Wash Buffer;High Salt Wash Buffer;LiCl Wash Buffer;TE Buffer;RIP Elution Buffer;10% SDS;0.5 M EDTA;DNase I (RNase Free);DNase I Supplement;DNase I Reaction Buffer ;Protease Inhibitor Cocktail III, Animal Free ;RNAse Inhibitor;Proteinase K
Jogi információk
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Tárolási osztály kódja
10 - Combustible liquids
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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