跳转至内容
Merck

Pulmonary pericytes regulate lung morphogenesis.

Nature communications (2018-06-24)
Katsuhiro Kato, Rodrigo Diéguez-Hurtado, Do Young Park, Seon Pyo Hong, Sakiko Kato-Azuma, Susanne Adams, Martin Stehling, Britta Trappmann, Jeffrey L Wrana, Gou Young Koh, Ralf H Adams
摘要

Blood vessels are essential for blood circulation but also control organ growth, homeostasis, and regeneration, which has been attributed to the release of paracrine signals by endothelial cells. Endothelial tubules are associated with specialised mesenchymal cells, termed pericytes, which help to maintain vessel wall integrity. Here we identify pericytes as regulators of epithelial and endothelial morphogenesis in postnatal lung. Mice lacking expression of the Hippo pathway components YAP and TAZ in pericytes show defective alveologenesis. Mutant pericytes are present in normal numbers but display strongly reduced expression of hepatocyte growth factor leading to impaired activation of the c-Met receptor, which is expressed by alveolar epithelial cells. YAP and TAZ are also required for expression of angiopoietin-1 by pulmonary pericytes, which also controls hepatocyte growth factor expression and thereby alveologenesis in an autocrine fashion. These findings establish that pericytes have important, organ-specific signalling properties and coordinate the behavior of epithelial and vascular cells during lung morphogenesis.

材料
货号
品牌
产品描述

Sigma-Aldrich
脱氧核糖核酸酶 I 来源于牛胰腺, lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein
Sigma-Aldrich
红细胞裂解缓冲液Hybri-Max, liquid, sterile-filtered, suitable for hybridoma
Sigma-Aldrich
明胶 来源于猪皮肤, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
Sigma-Aldrich
蓖麻油,乙氧基化, pH range 6.0-8.0
Sigma-Aldrich
单克隆抗-肌动蛋白,α-平滑肌, clone 1A4, ascites fluid
Sigma-Aldrich
抗-肌动蛋白, α-平滑肌- Cy3抗体,小鼠单克隆, clone 1A4, purified from hybridoma cell culture
Sigma-Aldrich
环己酰亚胺,大包装, ≥90% (HPLC)
Sigma-Aldrich
抗NG2硫酸软骨素蛋白聚糖抗体, Chemicon®, from rabbit
Sigma-Aldrich
抗-Prosurfactant蛋白C(proSP-C)抗体, serum, Chemicon®
Sigma-Aldrich
多聚甲醛, reagent grade, crystalline
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)
Sigma-Aldrich
抗-WWTR1 兔抗, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
2-羟乙基琼脂糖
Sigma-Aldrich
抗水通道蛋白5兔pAb, liquid, Calbiochem®