推荐产品
产品名称
红细胞裂解缓冲液Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
无菌性
sterile-filtered
表单
liquid
技术
cell culture | hybridoma: suitable
杂质
endotoxin, tested
应用
建议在细胞颗粒中加入1 mL缓冲液(细胞颗粒=1个脾脏或1-2亿个细胞)。 轻轻混合1分钟。 用15-20mL培养基活盐溶液稀释该缓冲液。 在250-500 × g 离心7分钟并弃去上清。 细胞可被稀释并准备计数或融合。 如果裂解不完全，可重复1-4步。
生化/生理作用
红细胞裂解缓冲液已被开发用于杂交瘤实验中，用于在融合前从小鼠脾细胞悬液中去除红细胞。 在需要从细胞悬液(如全血)中去除红细胞的体系中，它也很有用。
组分
含8.3 g/L 氯化铵的0.01 M Tris-HCl 缓冲液。
其他说明
注：本品用于去除小鼠红细胞。 本品可能不适用于其他动物红细胞的裂解。 该产品在小鼠脾细胞以外的任何应用场合的适用性必须由研究人员确定。
法律信息
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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