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Merck

53567-U

Supelco

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 2.1 mm, HPLC Column

别名:

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express F5, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

材料

stainless steel column

agency

suitable for USP L43

產品線

Ascentis®

特點

endcapped

製造商/商標名

Ascentis®

包裝

1 ea of

參數

60 °C temp. range
600 bar max. pressure (9000 psi)

技術

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

長度 × 內徑

5 cm × 2.1 mm

表面積

135 m2/g

雜質

<5 ppm metals

基質

Fused-Core particle platform
superficially porous particle

基質活性組

PFP (pentafluorophenyl) phase

粒徑

2.7 μm

孔徑

90 Å pore size

工作pH值

2-8

應用

food and beverages

分離技術

reversed phase

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相关类别

一般說明

由于存在电负性氟,Ascentis Express F5 的五氟苯基丙基固定相可提供具有缺电子苯环的稳定反相填料。除了形成 pi-pi 和温和的空间相互作用外,F5 相也通过极性相互作用保留化合物。Ascentis Express F5 可用于碱性、酸性或中性化合物,选择性与 C18 不同。

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

商品

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

实验方案

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

Chromatograms

application for HPLC

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