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用途
kit sufficient for 1,000 tests
包裝
pkg of 1 kit
儲存條件
dry at room temperature
λmax
570 nm
應用
cell analysis
detection
檢測方法
colorimetric
儲存溫度
2-8°C
一般說明
本试剂盒旨在利用活细胞的线粒体活性功能通过比色法测定细胞数/细胞活力。MTT法是一种简便、准确、结果可重复的方法。关键组分是(3-[4,5-二甲基-2-噻唑基]-2,5-二苯基溴化四唑) 或MTT。溶于无酚红的培养基或平衡盐溶液中的MTT溶液呈浅黄色。其四唑环可被活细胞的线粒体脱氢酶剪切而产生不溶于水溶液的紫色甲臜晶体。在570nm波长处测量转化染料的吸光度。
應用
MTT体外细胞毒性检测试剂盒已用于通过(3-[4,5-二甲基-2-噻唑基]-2,5-二苯基溴化四唑)MTT法检测金纳米颗粒(AuNP)的细胞毒性。它还用于检测SH-SY5Y培养物经过6-羟基多巴胺(6-OHDA)处理后的细胞活性。
生化／生理作用
将MTT转化成水不溶性的有色甲臜衍生物，然后用酸性异丙醇溶解。
訊號詞
Danger
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3
標靶器官
Central nervous system, Respiratory system
儲存類別代碼
3 - Flammable liquids
閃點（°F）
53.6 °F
閃點（°C）
12 °C
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J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
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商品
用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖检测（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，用于癌症、神经科学和干细胞研究应用。
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