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包装
pkg of 1 kit
技术
UV/Vis spectroscopy: suitable
protein quantification: suitable
Amax
570 nm
应用
cell analysis
detection
检测方法
colorimetric
运输
dry ice
储存温度
−20°C
一般描述
细胞生长测定试剂盒（基于MTT）设计用于以分光光度法测量细胞生长，作为活细胞中线粒体活性的函数。MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)系统是一种简单、准确、可重复性的方法，可通过线粒体脱氢酶活性测量活细胞活性。
应用
细胞生长测定试剂盒（基于MTT）已用于：
- 测定成骨前细胞的细胞活力
- 正常人前列腺间质和上皮细胞的MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯溴化四唑)检测
- 测定原代宫颈上皮细胞的细胞活力
包装
每个试剂盒包括5瓶试剂，每瓶含1 mL 5 mg/ml MTT的RPMI溶液（无酚红）和50 mL MTT溶剂（0.1N盐酸的无水异丙醇溶液）。 优化后的各组分体积大小尤其适合多孔板细胞生长。 MTT溶液按总培养体积的10%添加，因此每个试剂盒的测试次数取决于培养容量和/或体积大小。
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警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
靶器官
Central nervous system
储存分类代码
3 - Flammable liquids
闪点(°F)
53.6 °F - closed cup
闪点(°C)
12 °C - closed cup
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商品
用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
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