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Merck

TOX6

Sigma-Aldrich

体外毒理学检测试剂盒,基于磺酰罗丹明B

别名:

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84

形狀

liquid

用途

 kit sufficient for 1,000 tests

包裝

pkg of 1 kit

儲存條件

dry at room temperature

λmax

565 nm

應用

cell analysis
detection

檢測方法

colorimetric

儲存溫度

room temp

一般說明

磺酰罗丹明B方法简便、准确,结果一致。该分析系统是一种测量总生物量的方法,通过染色细胞蛋白与硫血红蛋白B。

應用

基于磺酰罗丹明B的体外毒性检测试剂盒已被用于通过总蛋白进行生物质(活细胞和死细胞)的分光光度测定。它用于研究17-烯丙基氨基-17-去甲氧格尔德霉素 (17-AAG) 对五种葡萄膜黑色素瘤细胞系增生的作用。在565nm波长处测量染料的吸光度。

生化/生理作用

染料与细胞蛋白结合并随后溶解在碱中。

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

儲存類別代碼

3 - Flammable liquids


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Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
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Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
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Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
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商品

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

基于细胞的细胞增殖检测(BrdU、MTT、WST1)、细胞活力和细胞毒性实验,用于癌症、神经科学和干细胞研究应用。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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