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Merck

T7818

Sigma-Aldrich

®缓冲剂

BioPerformance Certified, pH 7.5, average Mw 150.6

别名:

Tris 晶体

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

BioPerformance Certified

形狀

crystalline powder

分子量

average Mw 150.6

技術

cell culture | mammalian: suitable

雜質

DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH值

7.50 (25 °C, 0.1 M)(lit.)
7.5

有用的pH值範圍

7.0-9.0

溶解度

water: 0.66 g/mL, clear, colorless

應用

diagnostic assay manufacturing

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應用

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。

其他說明

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
某些pH电极不能使用Tris缓冲液提供准确的结果。

準備報告

Trizma溶液可高压灭菌。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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