T0319

^®缓冲剂

BioPerformance Certified, pH 7.4, average M_w 151.6

• 别名: Tris 晶体
• 分類程式碼代碼: 12161700
• NACRES: NA.25

属性

• 等級: BioPerformance Certified
• 形狀: crystalline powder
• 分子量: average M_w 151.6
• 技術: cell culture | mammalian: suitable
• 雜質: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH值: 7.12 (37 °C, 0.1 M)(lit.); 7.40 (25 °C, 0.1 M)(lit.); 7.4; 7.97 (5 °C, 0.1 M)(lit.)
• 有用的pH值範圍: 7.0-9.0
• 溶解度: deionized water: soluble at 25 °C, clear, colorless (pH 7.4, 1 pouch dissolved in 1 L to yield a 0.1 M Trizma solution)
• 應用: diagnostic assay manufacturing

说明

應用

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。

包裝

简易锡箔袋包装

其他說明

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

某些pH电极不能使用Tris缓冲液提供准确的结果。

準備報告

Trizma溶液可高压灭菌。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: dust mask type N95 (US), Eyeshields, Gloves