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Merck
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主要文件

SRE0055

Sigma-Aldrich

蛋白酶抑制剂粉

Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.

别名:

蛋白酶抑制剂混合物粉末

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

bovine lung
yeast

表单

lyophilized powder

储存温度

−20°C

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特异性

抑制丝氨酸、半胱氨酸和金属蛋白酶。

应用

已针对一般用途进行了优化和测试。
蛋白酶抑制剂混合物粉末已用于免疫印迹和免疫沉淀中。

生化/生理作用

该混合物含有单独的组分,包括2mM的AEBSF、0.3 μ的抑肽酶、116 μM的乌苯美司、14 μM的E-64、1 μM的亮肽素和1mM的EDTA。每种组分都具有特定的抑制特性。

AEBSF和抑肽酶可以抑制丝氨酸蛋白酶,包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。乌苯美司可抑制氨肽酶。E-64可抑制半胱氨酸蛋白酶。EDTA是一种金属蛋白酶的抑制剂。亮肽素作用于丝氨酸和半胱氨酸蛋白酶。
蛋白酶有助于将蛋白质分解成可溶性肽和氨基酸。

组分

1瓶血清瓶
组分
AEBSF
抑肽酶
乌苯美司
E-64
EDTA
亮抑酶肽

数量

一瓶可制备100mL混合液,其足以用于抑制约20克细胞提取物中的蛋白酶。
并非所有提取物都含有相同水平的内源酶,并且可能需要调整所需的混合液用量。

制备说明

该产品以冻干粉形式提供。一瓶可制备100毫升混合液。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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