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Merck
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MSSAFE

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.

别名:

质谱安全蛋白酶和磷酸酶抑制剂混合物

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.77

product name

MS-SAFE蛋白酶和磷酸酶抑制剂,

描述

for use with mammalian cell and tissue extract, lyophilized powder

形狀

powder

溶解度

water: 0.1 g/mL, clear, colorless

儲存溫度

2-8°C

相关类别

一般說明

质谱（MS）兼容蛋白酶抑制剂鸡尾酒和磷酸酶抑制剂鸡尾酒，具有广泛的抑制特异性：
  • 丝氨酸、半胱氨酸和天冬氨酸蛋白酶以及金属蛋白酶
  • 酪氨酸、丝氨酸/苏氨酸、酸性和碱性磷酸酶
选择所有抑制剂和填充剂，以免干扰LC-MS分析。避免了任何能够共价、不可逆蛋白质修饰的抑制剂。这种鸡尾酒不含EDTA。所有抑制剂的选择都是为了便于下游样品处理，例如用于His标记蛋白纯化的固定化金属亲和色谱（IMAC），以及独特的磷酸肽富集。

特異性

蛋白酶抑制剂：丝氨酸、半胱氨酸和天冬氨酸蛋白酶以及金属蛋白酶。
磷酸酶抑制剂：酪氨酸、丝氨酸/苏氨酸、酸性和碱性磷酸酶

應用

MS-SAFE蛋白酶和磷酸酶抑制剂已用于测量核糖体蛋白S6激酶（S6K）活性。
在哺乳动物细胞裂解物和肝组织提取物中测试。设计用于质谱分析的样本。

特點和優勢

用于质谱分析的综合蛋白酶和磷酸酶抑制剂混合物

与下游样品处理兼容，如 His 标记的蛋白纯化和磷酸肽富集

允许精确测量蛋白活性和鉴定磷酸化位点。

成分

蛋白酶抑制剂：
  • 贝他定盐酸
  • 亮抑酶肽
  • 磷酰胺基二钠盐
  • 胃蛋白酶抑制剂
  • 弹性酶抑制剂
  • 抑肽酶
  • 甲磺酸萘福莫司他
  • 抗蛋白酶
磷酸酶抑制剂
  • 豆渣酸
  • 氟化钠
  • 原钒酸钠
  • 草酸溴四胺酯
填料
  • β-乳糖
  • DL-亮氨酸

數量

一小瓶使用水或提取/裂解缓冲液制备20 mL 1倍抑制剂鸡尾酒工作溶液。或者，可以通过加入2 mL水或提取/裂解缓冲液来制备10倍的浓缩液。然后，根据1倍工作溶液的需要，可以将该10倍溶液10倍稀释到提取/裂解缓冲液中。

外觀

水溶性冻干粉末

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H302,H315,H319

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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S K Sreedharan et al.
The Biochemical journal, 316 ( Pt 3), 777-786 (1996-06-15)
1-trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane (E-64) was shown to inhibit beta-trypsin by a reversible competitive mechanism; this contrasts with the widely held view that E-64 is a class-specific inhibitor of the cysteine proteinases and reports in the literature that it does not inhibit a
Michael J Workman et al.
International journal of molecular sciences, 22(1) (2021-01-06)
Human intestinal organoids (HIOs) are increasingly being used to model intestinal responses to various stimuli, yet few studies have confirmed the fidelity of this modeling system. Given that the interferon-gamma (IFN-γ) response has been well characterized in various other cell
Chandresh R Gajera et al.
Journal of neuroscience methods, 312, 73-83 (2018-11-23)
Synaptic alterations, especially presynaptic changes, are cardinal features of neurodegenerative diseases and strongly correlate with cognitive decline. We report "Mass Synaptometry" for the high-dimensional analysis of individual human synaptosomes, enriched nerve terminals from brain. This method was adapted from cytometry
Xuming Wang et al.
Molecular medicine reports, 11(5), 3375-3381 (2014-12-30)
The present study investigated the protein expression levels of cluster of differentiation (CD)95, caspase‑8, caspase‑3 and poly(ADP‑ribose) polymerase 1 (PARP1) in liver cancer and its association with clinical pathological parameters. The results demonstrated that the expression of CD95 correlated with
Nina E Murugina et al.
The Journal of biological chemistry, 295(10), 3099-3114 (2020-02-02)
Upon activation with pathogen-associated molecular patterns, metabolism of macrophages and dendritic cells is shifted from oxidative phosphorylation to aerobic glycolysis, which is considered important for proinflammatory cytokine production. Fragments of bacterial peptidoglycan (muramyl peptides) activate innate immune cells through nucleotide-binding
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Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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