推荐产品
产品名称
SIGMAFAST ™蛋白酶抑制剂混合物片剂,无EDTA, for use in purification of Histidine-tagged proteins
生物源
bovine lung
品質等級
形狀
tablet
儲存溫度
2-8°C
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一般說明
SIGMAFAST™ 蛋白酶抑制剂混合液片,EDTA-Free是一种水溶性片剂配方,旨在抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。 该片剂不含强金属螯合剂,如EDTA,并与固定化金属亲和层析(IMAC)样品处理兼容。 该片剂还含有独特的专利配方,包括胃酶抑素A盐,以提供额外的保护。
特異性
抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。
應用
SIGMAFAST ™蛋白酶抑制剂混合液片的广泛特异性 使其适合用于组氨酸标记蛋白的纯化, 并作为通用蛋白酶抑制剂混合液。 该片剂还可以用作细胞裂解缓冲液的组件, 并已与HIS-Select介质一起使用进行了优化和测试。
特點和優勢
EDTA-Free: 不含可能干扰IMAC处理的强金属螯合剂,如EDTA。
胃酶抑素A:含有独特的专有配方(包括胃抑素A盐),可提供片剂形式通常无法提供的额外保护作用。
广泛特异性:抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶,为蛋白质样品提供广泛的保护。
高产率:一片可制成100 mL混合液,推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
长期稳定性:该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液(1´或10´)在2–8 °C下至少可稳定保存2周。
胃酶抑素A:含有独特的专有配方(包括胃抑素A盐),可提供片剂形式通常无法提供的额外保护作用。
广泛特异性:抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶,为蛋白质样品提供广泛的保护。
高产率:一片可制成100 mL混合液,推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
长期稳定性:该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液(1´或10´)在2–8 °C下至少可稳定保存2周。
成分
AEBSF
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
數量
一片可制备100 mL混合液。 推荐将一片用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
外觀
片剂是可溶于水的
儲存和穩定性
该片剂在2-8°C温度下至少可稳定保存2年。重悬的蛋白酶抑制剂溶液(1´或10´)在2–8°C下至少可稳定保存2周。
法律資訊
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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相關產品
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Corr. 1A
儲存類別代碼
8A - Combustible corrosive hazardous materials
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
历史批次信息供参考:
分析证书(COA)
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