推荐产品
生物源
bovine pancreas
品質等級
種類
Type I-A
形狀
powder
比活性
≥50 Kunitz units/mg protein
分子量
~13,700
濃度
≥60% (RNase A, SDS-PAGE)
技術
cell based assay: suitable
雜質
salt, essentially free
適合性
suitable for molecular biology
應用
diagnostic assay manufacturing
異物活動
protease, essentially free
儲存溫度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI 密鑰
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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一般說明
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
應用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
生化／生理作用
核糖核酸酶A是一种内切核糖核酸酶，可在嘧啶核苷酸后切割单链RNA。 它在3'磷酸基末端进行攻击。 核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNA酶还可以水解蛋白质样品中的RNA。 RNase A可被His12和His119的烷基化所抑制并被钾盐和钠盐所活化。
特點和優勢
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
準備報告
盐分级和色谱纯化。
分析報告
蛋白测定方法：E.
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Yang Han et al.
eLife, 7 (2018-08-25)
Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise
Elizaveta Katorcha et al.
PLoS pathogens, 14(6), e1007093-e1007093 (2018-06-22)
The main risk of emergence of prion diseases in humans is associated with a cross-species transmission of prions of zoonotic origin. Prion transmission between species is regulated by a species barrier. Successful cross-species transmission is often accompanied by strain adaptation
D Joseph-McCarthy et al.
Protein engineering, 9(9), 773-780 (1996-09-01)
One relatively new computational approach to the drug discovery process involves calculating functional group maps of a target structure. Experimental functional group mapping techniques have also recently emerged. In this paper, the structure of RNase A with two bound formates
Franziska Büscheck et al.
World journal of urology, 39(3), 829-837 (2020-05-04)
DNA ploidy measurement has earlier been suggested as a potentially powerful prognostic tool in many cancer types, but the role in renal tumors is still unclear. To clarify its prognostic impact, we analyzed the DNA content of 1320 kidney tumors
Barbara Schöpf et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(6), 1895-1900 (2012-01-11)
Single strand nicks and gaps in DNA have been reported to increase the efficiency of nucleosome loading mediated by chromatin assembly factor 1 (CAF-1). However, on mismatch-containing substrates, these strand discontinuities are utilized by the mismatch repair (MMR) system as
实验方案
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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