推荐产品
生物源
bovine pancreas
種類
Type XII-A
化驗
≥90% (SDS-PAGE)
形狀
lyophilized powder
比活性
75-125 Kunitz units/mg protein
分子量
~13,700
技術
cell based assay: suitable
雜質
salt, essentially free
適合性
suitable for mRNA or total RNA extracted from cells and tissues
應用
diagnostic assay manufacturing
異物活動
protease, essentially free
儲存溫度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI 密鑰
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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一般說明
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
應用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
生化／生理作用
核糖核酸酶A是一种内切核糖核酸酶，可在嘧啶核苷酸后切割单链RNA。它在3'磷酸基末端进行攻击。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNA酶还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化所抑制并被钾盐和钠盐所活化。
特點和優勢
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
準備報告
盐分级和色谱纯化。
分析報告
蛋白测定方法：E.
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Amy B Emerman et al.
Methods in molecular biology (Clifton, N.J.), 1413, 303-324 (2016-05-20)
RNAs associate with the mitotic spindle in a variety of organisms, where they can spatially regulate protein production, ensure their proper segregation during cell division, or perform translation-independent roles in spindle formation. The identification of spindle-associated RNAs is an important
Vlad Zabrouskov et al.
Biochemistry, 45(3), 987-992 (2006-01-18)
Although deamidation at asparagine and glutamine has been found in numerous studies of a variety of proteins, in almost all cases the analytical methodology that was used could detect only a single site of deamidation. For the extensively studied case
Amaya Albalat et al.
Methods in molecular biology (Clifton, N.J.), 984, 153-165 (2013-02-07)
The analysis of proteins and peptides in biological fluids is becoming more important as they are potential sources of diagnostic biomarkers of disease. The complexity of body fluids is such that no single technique can both identify and quantify all
Xin-Miao Fu et al.
Biochimica et biophysica acta, 1814(4), 487-495 (2011-01-18)
Protein disulfide isomerase (PDI) and its pancreatic homolog (PDIp) are folding catalysts for the formation, reduction, and/or isomerization of disulfide bonds in substrate proteins. However, the question as to whether PDI and PDIp can directly attack the native disulfide bonds
Romina Ponzielli et al.
Nucleic acids research, 36(21), e144-e144 (2008-10-23)
High-throughput, microarray-based chromatin immunoprecipitation (ChIP-chip) technology allows in vivo elucidation of transcriptional networks. However this complex is not yet readily accessible, in part because its many parameters have not been systematically evaluated and optimized. We address this gap by systematically
实验方案
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
本实验方案可用于测定核糖核酸酶A(RNase A)的活性。
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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