推荐产品
製造商／商標名
Novagen®
儲存條件
OK to freeze
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
ProteoExtract®跨膜蛋白质提取试剂盒（TM-PEK）提供了无洗涤剂的新型化学方法，可从哺乳动物细胞和组织中温和有效地提取跨膜蛋白，例如GPCR。膜蛋白中富含的级分和大蛋白复合物与酶测定、非变性和基于SDS的凝胶电泳、蛋白质印迹和免疫沉淀直接兼容。富含的级分适用于胰蛋白酶凝胶内消化后基于质谱的分析。该试剂盒包含用于蛋白质依赖性优化的两种不同提取试剂、蛋白酶抑制剂混合物和用于处理二十个样品的适当缓冲液（每个样品2-5 x 106个细胞或25-50 mg组织）。
應用
ProteoExtract®跨膜蛋白提取试剂盒已用于： 提取质膜蛋白的Na+/H+交换剂（NHE3）的蛋白质印迹分析
特點和優勢
- 不需要超声、强涡流、长时间的超离心或高温孵育。
- 试剂足以处理总共40个样品。
警告
毒性：有毒（F）
其他說明
Nakamura，E.，et al. 2008.Cancer Sci.99， 1390.
法律資訊
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
Impairment of electroneutral Na+ transport and associated downregulation of NHE3 contributes to the development of diarrhea following in vivo challenge with Brachyspira spp.
Cole B. Enns, et al.
The American Journal of Physiology, 318(2), G288?G297-G288?G297 (2020)
Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters
Liu L, et al.
The American Journal of Pathology, 187(12), 2775-2787 (2017)
Runtao Ding et al.
Frontiers in behavioral neuroscience, 14, 60-60 (2020-05-05)
The monocarboxylate transporters (MCTs) MCT1, MCT2, and MCT4 are essential components of the astrocyte-neuron lactate shuttle (ANLS), which is a fundamental element of brain energetics. Decreased expression of MCTs can induce cognitive dysfunction of the brain. In the present study
Huimin Luo et al.
Oncology reports, 42(3), 1237-1247 (2019-07-20)
Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx
Runtao Ding et al.
Scientific reports, 6, 38771-38771 (2016-12-10)
Ketamine has been reported to impair human cognitive function as a recreational drug of abuse. However, chronic effects of ketamine on central nervous system need to be further explored. We set out to establish chronic ketamine addiction models by giving
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