推荐产品
品質等級
形狀
lyophilized powder
技術
DNA extraction: suitable
適合性
suitable for microbiology
應用
microbiology
運輸包裝
wet ice
儲存溫度
-10 to -25°C
一般說明
A common difficulty among microbiome scientists is to extract nucleic acids from difficult to lyse microbes that form capsules or highly resistant spores. Some microbes are difficult to disrupt because the cell walls may form capsules or resistant spores. To address this challenge, protocols that integrate lysing enzymes can be incorporated into the nucleic acid extraction workflow.
應用
Isolating RNA from microbiome samples poses many challenges. RNA molecules are highly labile and are prone to degradation by contaminating RNases. As such, to adapt the MetaPolyzyme for RNA isolation, a broad range RNase inhibitor has been incorporated in order to help prevent RNA degradation by various RNases. MAC4LRI, includes all 6 enzymes from MetaPolyzyme to ensure maximal lysis of microorganisms while protecting the sample RNA from degradation. While it is not possible to inhibit all RNases from a specific microbial environment, we haver incorporated a proprietary RNase Inhibitor to help inhibit as many RNases as possible.
The MetaPolyzyme product family includes MAC4L, MAC4LDF (DNA-Free), SAEO200 (MycoPolyzme, for yeast and fungal lysis, DNA-Free) and MAC4LRI (RNase Inhibitor) and are based on a multi-lytic enzyme mixture formulated for effective lysis of microbiome samples from any sample type. Originally, MetaPolyZyme was developed by Scott Tighe for use on samples from extreme environments and general applications to increase DNA extraction efficiency for any method. To date, these products have been further evaluated and continuously developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG; formerly the Metagenomics Research Group, MGRG). Several publications have cited use of MetaPolyzyme, suggesting various advantages to the usage of MetaPolyzyme for enhancing bacterial lysis.
The MetaPolyzyme product family includes MAC4L, MAC4LDF (DNA-Free), SAEO200 (MycoPolyzme, for yeast and fungal lysis, DNA-Free) and MAC4LRI (RNase Inhibitor) and are based on a multi-lytic enzyme mixture formulated for effective lysis of microbiome samples from any sample type. Originally, MetaPolyZyme was developed by Scott Tighe for use on samples from extreme environments and general applications to increase DNA extraction efficiency for any method. To date, these products have been further evaluated and continuously developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG; formerly the Metagenomics Research Group, MGRG). Several publications have cited use of MetaPolyzyme, suggesting various advantages to the usage of MetaPolyzyme for enhancing bacterial lysis.
特點和優勢
- Disrupts difficult to lyse microbe samples
- Protects RNA from degradation
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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