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溶葡球菌酶 来源于溶葡萄球菌, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein
生物源
microbial (Staphylococcus staphylotyticus)
形狀
lyophilized powder
比活性
≥500 units/mg protein
分子量
27 kDa
特點
DNA free
運輸包裝
wet ice
儲存溫度
−20°C
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應用
从葡萄球菌SAE0091中纯化的细胞外溶葡萄球菌素已经过严格的质量控制测试,以确保通过35个循环对16S和18S rDNA(使用通用引物对)进行PCR扩增时,不存在可检测水平的污染DNA。
溶葡萄球菌酶是从溶葡萄球菌的细菌培养物中分离出的锌金属酶。它对其他葡萄球菌(包括金黄色葡萄球菌)具有特异性裂解作用。1,2溶葡萄球菌素具有
己糖胺酶、酰胺酶和内肽酶活性。它裂解细胞壁中的聚甘氨酸交联,导致葡萄球菌物种的细胞裂解,但不导致其他细菌属的细胞裂解。[Pharmaceuticals 2010, 3(4), 1139-1161]
溶葡萄球菌素是一条包含246个氨基酸的多肽链,分子量为26,926 Da,等电点为9.5,(5),最适pH值为7.5。(6)
溶葡萄球菌酶是从溶葡萄球菌的细菌培养物中分离出的锌金属酶。它对其他葡萄球菌(包括金黄色葡萄球菌)具有特异性裂解作用。1,2溶葡萄球菌素具有
己糖胺酶、酰胺酶和内肽酶活性。它裂解细胞壁中的聚甘氨酸交联,导致葡萄球菌物种的细胞裂解,但不导致其他细菌属的细胞裂解。[Pharmaceuticals 2010, 3(4), 1139-1161]
溶葡萄球菌素是一条包含246个氨基酸的多肽链,分子量为26,926 Da,等电点为9.5,(5),最适pH值为7.5。(6)
生化/生理作用
溶葡萄球菌素是一种分子量约为25 kDa的锌内肽酶。由于溶葡萄球菌酶能裂解 葡萄球菌 类细胞壁肽聚糖层中的聚甘氨酸交联键,它已被发现可用于细胞裂解,并具有潜在的抗菌治疗作用。活性的最优pH值:~7.5。
溶葡萄球菌素是一种分子量约为25 kDa的锌内肽酶。 由于溶葡萄球菌酶能裂解 葡萄球菌 类细胞壁肽聚糖层中的聚甘氨酸交联键,它已被发现可用于细胞裂解,并具有潜在的抗菌治疗作用。
活性最佳pH:约 7.5
活性最佳pH:约 7.5
單位定義
一个单位在25℃和pH 7.5条件下,在6.0 ml反应混合物中可使金黄色葡萄球菌细胞混悬液的浊度(A620)在10分钟内由0.250降至0.125。
外觀
以冻干粉形式提供,其中含有50-70%的蛋白质,其余主要为NaCl。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
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Frontiers in cellular and infection microbiology, 6, 187-187 (2017-01-14)
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商品
裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程:只需加入酶,将试管放入水浴然即可。但这过程中到底发生了什么?
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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