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Merck

MAK466

Sigma-Aldrich

Free Fatty Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

應用

agriculture
food and beverages
pharmaceutical

檢測方法

colorimetric
fluorometric

相關疾病

diabetes; pediatric diseases; endocrinological disorders; neurological disorders

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Fatty acids are aliphatic monocarboxylic acids ubiquitously found in animal or vegetable fat, oil, and wax. Fatty acids play important roles in cellular synthesis, energy metabolism, and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome, and Reye Syndrome.

特點和優勢

  • Linear detection range: colorimetric assay 7 - 1000 µM, fluorometric assay 7 - 100 µM fatty acid.
  • Compatible with high-throughput handling systems.

適合性

The kit is suitable for detecting free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, and cell cultures, and in food and agriculture products, as well as studying the effects of drugs on free fatty acid metabolism.

原則

The Free Fatty Acid Assay Kit provides a simple, one-step and high-throughput assay for measuring Free Fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity (λEx = 530 nm/λEm = 585 nm) is directly proportional to the free fatty acid concentration in the sample.

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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