LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• 别名: Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• EC 号: 3.4.21.50 (BRENDA, IUBMB)
• 分類程式碼代碼: 12352204
• NACRES: NA.54

属性

• 生物源: Achromobacter lyticus
• 品質等級: 200
• 重組細胞: expressed in E. coli (206-473aa)
• 等級: Proteomics Grade
• 形狀: ready-to-use solution
• 濃度: 1 mg/mL
• 最適pH: 8.5
• pH值範圍: 9.0-9.5(catalytic)
• UniProt登錄號: P15636
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

特異性

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

應用

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

包裝

1mg/ml in Plastic

準備報告

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

儲存和穩定性

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

其他說明

For R&D only.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable