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Merck

LY0100

Sigma-Aldrich

溶菌酶活性试剂盒

sufficient for 100 tests

别名:

Lysozyme assay kit

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.26

用途

sufficient for 100 tests

品質等級

儲存溫度

2-8°C

一般說明

溶菌酶检测试剂盒提供检测溶菌酶活性存在的即用型试剂。该检测溶菌酶活性的简单方法使用 溶壁微球菌细胞作为底物。溶菌酶活性导致溶壁微球菌细胞裂解。在孵育溶菌酶样品和底物期间,通过监测 450 nm 处的吸光度降低来跟踪反应。

應用

溶菌酶活性试剂盒已用于测定天然溶菌酶、鸡蛋清纯化的溶菌酶和毛果芸香碱刺激唾液中溶菌酶的催化活性。

生化/生理作用

溶菌酶水解裂解 N -乙酰-D-氨基葡萄糖和 N -乙酰胞壁酸残基(GlcNAcβ1-4Mur)的粘多糖和相应的肽聚糖的聚糖骨架(后者是通过肽链连接或交联的糖胺聚糖)。溶菌酶能有效地破坏细菌的细胞壁。

儲存和穩定性

反应缓冲液在 2–8°C 下至少可稳定 2 年。
溶菌酶(目录号 L6876)可在 –20℃ 下长期储存,并保持活性 4 年。
溶壁微球菌细胞(目录号 M3770)应在-20°C 条件下长期储存。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • L6876Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg protein化学品安全说明书

  • Lysozyme Reaction Buffer

  • M3770Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cells化学品安全说明书

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification of lysozyme (LYZ), directly from chicken egg white (EW). The Tris-cryogel presented
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