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生物源
chicken egg white
形狀
liquid
分子量
single-chain 14.3 kDa
濃度
≥400000 units/mL
10 mg/mL
技術
cell based assay: suitable
適合性
suitable for cell lysis
UniProt登錄號
應用
cell analysis
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
chicken ... LYZ(396218)
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一般說明
溶酶体是一种存在于蛋清中的酶，它能分解细菌细胞壁，提高提取 DNA 和蛋白质的效率。
應用
适用于细菌 DNA 和蛋白质的纯化。
酶可分解细菌的细胞壁；用于制备原生质体。
生化／生理作用
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。
该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
適合性
采用 CelLytic ™ B 从细菌中提取蛋白质的适用性试验。
單位定義
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
外觀
50% 甘油、25 mM 醋酸钠溶液
即用型溶液：10 mg/mL溶菌酶，溶于含50%甘油的25 mM醋酸钠（pH 4.5）中。
法律資訊
CelLytic is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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实验方案
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
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