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应用
CelLytic ™ B 细胞裂解试剂已用于细胞裂解，用于提取辅酶 Q10、 重组 Src 激酶相关磷蛋白 1-谷胱甘肽 S -转移酶 (GST) 融合蛋白 和人 α-突触核蛋白。
两性离子洗涤剂的专利、非变性配方，用于细菌细胞裂解和重组蛋白提取。
特点和优势
- 比传统方法如超声法和溶菌酶法提取蛋白质效率高
- 可放大 1-25 克细菌细胞浆
- 不干扰下游应用，如亲和层析、IP 和免疫印迹
- 与蛋白酶抑制剂、抑制剂混合物、离液剂、盐、螯合剂、还原剂相容
其他说明
在 40 mM Trizma ® HCl (pH 8.0) 中配制的洗涤剂混合物。
法律信息
此产品受美国专利号7,282,475 B2管辖，仅针对研究用途出售。如需商业化使用，需另行征得许可。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens
Ha SJ, et al.
Bioprocess and Biosystems Engineering, 32(5), 697-700 (2009)
Yuki Okegawa et al.
Biochemistry and biophysics reports, 4, 148-151 (2015-09-11)
The seamless ligation cloning extract (SLiCE) method is a novel seamless DNA cloning tool that utilizes homologous recombination activities in Escherichia coli cell lysates to assemble DNA fragments into a vector. Several laboratory E. coli strains can be used as
Prion-like seeding of misfolded alpha-synuclein in the brains of dementia with Lewy body patients in RT-QUIC
Sano K, et al.
Molecular Neurobiology, 55(5), 3916-3930 (2018)
Kazunori Sano et al.
Acta neuropathologica communications, 9(1), 182-182 (2021-11-14)
Serine 129 (S129) phosphorylation of α-synuclein (αSyn) is a central feature of Lewy body (LB) disease pathology. Although the neighboring tyrosine residues Y125, Y133, and Y136 are also phosphorylation sites, little is known regarding potential roles of phosphorylation cross-talk between
Immune adaptor protein SKAP1 (SKAP-55) forms homodimers as mediated by the N-terminal region
Raab M, et al.
BMC Research Notes, 11(1), 869-869 (2018)
商品
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
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