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产品名称
StableCell™ IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
品質等級
無菌
sterile-filtered
形狀
liquid
技術
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
雜質
Endotoxin, tested
成分
NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
该 IMDM 与原处方不同,因为其添加了 L-丙氨酰-L-谷氨酰胺二肽。众所周知,谷氨酰胺在细胞培养中是不稳定的。这种二肽为细胞培养提供了一种更稳定的谷氨酰胺形式。
應用
经 Guilbert 和 Iscove 证明,红细胞和巨噬细胞的前体细胞可在添加白蛋白、转铁蛋白、卵磷脂和硒的完全确定的无血清培养基中培养。该培养基是对 Dulbecco 改良 Eagle 培养基 (DME) 的改良,含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES 缓冲液和硝酸钾而不是硝酸铁。进一步研究证明,Iscove 培养基可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞。
法律資訊
StableCell is a trademark of Sigma-Aldrich Co. LLC
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产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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