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Merck

G7509

Sigma-Aldrich

GABase 来源于荧光假单胞菌

lyophilized powder, Protein ≥30 % by biuret

别名:

GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase

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About This Item

MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

Pseudomonas fluorescens

品質等級

化驗

≥30  % (biuret)

形狀

lyophilized powder

比活性

≥0.5 units/mg protein

成份

Protein, ≥30% biuret

技術

inhibition assay: suitable

儲存溫度

−20°C

一般說明

研究领域:神经科学


GABase是一种市售酶混合物,由来自于荧光假单胞菌(Pseudomonas fluorescens)的γ-氨基丁酸氨基转移酶(GABGT)和琥珀酸半醛脱氢酶(SSDH)组成。

應用

来自荧光假单胞菌(Pseudomonas fluorescens)的GABase已被用于GABase测定,以测量GABA(γ-氨基丁酸)的细胞外和细胞内浓度。

生化/生理作用

GABase 催化中枢神经系统中的γ-氨基丁酸(GABA)转化为琥珀酸。它用于测定各种生物学样品中的GABA水平。对羟基苯甲醛(HBA)是潜在的GABase抑制剂。

單位定義

一个酶活性单位是指在pH 8.6、25°C下,每分钟将1.0 μmole的γ-氨基丁酸(GABA)转化为琥珀酸半醛、再转化为琥珀酸,并且化学计量还原1.0 μmole的NADP+所需的酶量。

外觀

部分纯化的冻干粉末,含有缓冲盐和稳定剂

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Mingu Gordon Park et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 2016-2024 (2021-09-14)
Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug
Kimon-Andreas G Karatzas et al.
Applied and environmental microbiology, 76(11), 3529-3537 (2010-04-20)
It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that under acidic conditions extracellular glutamate
Structural and Mechanistic Basis for the Inactivation of Human Ornithine Aminotransferase by (3 S,4 S)-3-Amino-4-fluorocyclopentenecarboxylic Acid
Shen S, et al.
Molecules (Basel), 28(3) (2023)
Nicolaie Moldovan et al.
Sensors and actuators. B, Chemical, 337 (2022-05-24)
Glutamate (GLU) and gamma-aminobutyric acid (GABA) are neurotransmitters (NTs) with an essential role in signal transmission in the brain. Brain disorders, such as epilepsy, Alzheimer's and Parkinson's diseases, and traumatic brain injury can be linked to imbalances in the GLU-GABA
A Vidal-Cros et al.
The Biochemical journal, 229(3), 675-678 (1985-08-01)
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis

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