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G7396

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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About This Item

CAS号:
9001-45-0
EC 号:
3.2.1.31 (BRENDA, IUBMB)
EC號碼:
232-606-8
MDL號碼:
MFCD00130629
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type IX-A

品質等級

300

形狀

lyophilized powder

比活性

1,000,000-5,000,000 units/g protein (30 min assay)

分子量

290 kDa

成份

Protein, 30-70% biuret

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

應用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。
用于尿液代谢物分析中葡糖苷酸结合物的水解

品質

含有缓冲盐和稳定剂。

單位定義

一个Sigma或改良Fishman单位是指在pH 6.8、温度37°C条件下（30分钟测定），每小时从苯酚葡萄糖醛酸中释放出1.0 μg酚酞所需的酶量。

也與該產品經常一起購買

产品编号
说明
价格

基底

产品编号
说明
价格

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000398556
0000398552
0000398557
0000398555
0000398558

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Maria Papageorgiou et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 35(10), 2032-2044 (2018-08-18)
Deoxynivalenol (DON) is a Fusarium toxin, to which humans are frequently exposed via diet. Although the elderly are speculated to be sensitive to the toxic effects of DON as a result of age-related conditions, disease and altered DON metabolism, there
Bojan Šarkanj et al.
Analytica chimica acta, 1019, 84-92 (2018-04-08)
There is a critical need to better understand the patterns, levels and combinatory effects of exposures we are facing through our diet and environment. Mycotoxin mixtures are of particular concern due to chronic low dose exposures caused by naturally contaminated
Enzymes
Hans Ulrich Bergmeyer
Methods of Enzymatic Analysis, 460-461 (1974)
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine
Health Laboratory Science, 50, 286-289 (2004)
Ping Wang et al.
Journal of analytical toxicology, 30(8), 570-575 (2006-11-30)
Confirmation of opioids in urine samples of clinical patients requires liberation of opioids from their glucuronide conjugates. Both acid hydrolysis and enzyme hydrolysis using beta-glucuronidase from various sources have been reported, with the latter approach prevailing in most clinical toxicology
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β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

使用UHPLC/MS（TOF）检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解，必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估，例如孵育时间、温度、水解pH、酶源和酶浓度。

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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