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EDEGLY

Sigma-Aldrich

酶蛋白去糖基化试剂盒

别名:

Glycosylation Release Kit

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

共軛

(N-linked)
 (O-linked)

品質等級

200

運輸包裝

wet ice

儲存溫度

2-8°C

相关类别

一般說明

酶蛋白去糖基化试剂盒包含从糖蛋白中完整去除N连接和单一O连接碳水化合物所需的所有酶和试剂。该试剂盒用于裂解Core 2 O连接碳水化合物复合物，包括含有聚乳糖胺的碳水化合物。

應用

酶促蛋白去糖基化试剂盒用于：
  • 蛋白质去糖基化
  • 组织因子途径抑制因子(TFPI)去糖基化α以及β
  • 天然exo-β-(1,3)-葡聚糖酶EXG1的去糖基化

酶解蛋白去糖基化试剂盒已用于各种酶的去糖基化。

特點和優勢

酶蛋白去糖基化试剂盒具有以下特点：
  • 适用于两mg以上规模的糖蛋白去糖基化
  • 中性pH值下单步反应
  • 天然&变性过程
  • 蛋白质无降解
  • 去除含聚唾液酸的O连接糖
  • 随附对照糖蛋白

仅试剂盒组分

产品编号
说明
  • PNGase F 1 mL/vial
  • O-Glycosidase 20 μL
  • α-2(3,6,8,9)-Neuraminidase 20 μL
  • Fetuin Control .5 mg
  • 5× Reaction Buffer .2 mL
  • Denaturation Solution .1 mL
  • Triton™ X-100 .1 mL
  • β-1→4-Galactosidase, positionally specific 20 μL
  • β-N-Acetylglucosaminidase 20 μL
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说明
价格

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families
Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)
Julien Chapuis et al.
Human molecular genetics, 20(10), 2026-2036 (2011-03-02)
Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by cerebral deposition of amyloid-β (Aβ), a series of peptides derived from the processing of the amyloid-β precursor protein (APP). To identify new candidate genes for AD, we recently performed
Jonathan Trapala et al.
Protein expression and purification, 176, 105718-105718 (2020-08-11)
Exo-inulinases are versatile enzymes that have gained attention in recent years due to their ability to hydrolyze linear and branched polyfructose chains found in inulines. Agavin, a branched inulin, is found in Agave plant, the raw matter to produce tequila.
Axel T Lehrer et al.
Vaccine, 36(22), 3090-3100 (2017-02-22)
Infections with filoviruses in humans are highly virulent, causing hemorrhagic fevers which result in up to 90% mortality. In addition to natural infections, the ability to use these viruses as bioterrorist weapons is of significant concern. Currently, there are no
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