A1054
抗-地高辛-碱性磷酸酶抗体，小鼠单克隆
clone DI-22, purified from hybridoma cell culture
别名:
单克隆抗-地高辛, 单克隆抗 地高辛 – 碱性磷酸酶 小鼠抗
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About This Item
分類程式碼代碼:
12352203
NACRES:
NA.46
共軛:
alkaline phosphatase conjugate
application:
DB
ELISA (d)
ELISA (d)
無性繁殖:
DI-22, monoclonal
技術:
direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
citations:
9
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生物源
mouse
品質等級
重組細胞
expressed in mouse cell line
共軛
alkaline phosphatase conjugate
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
DI-22, monoclonal
形狀
buffered aqueous solution
技術
direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
同型
IgG1
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
地高辛是一种强心苷，可用于治疗慢性心力衰竭。抗地高辛碱性磷酸酶单克隆抗体可用于检测地高辛标记的化合物，例如寡核苷酸、抗体和肽。该抗体对地高辛和地高辛标记的化合物具有特异性。该产品与地高辛具有很强的交叉反应性。
特異性
抗体对地高辛具有特异性，且对地高辛具有高亲和力。
免疫原
地高辛-KLH。
應用
巢式PCR产物可与5′端地高辛标记的寡核苷酸探针杂交。 向1 M磷酸盐缓冲液、1％牛血清白蛋白和0.1％吐温20（pH 7.4）中添加1:1000稀释的抗地高辛单克隆抗体碱性磷酸酶（DI-22）进行ELISA分析，以检测探针。使用Sigma Fass-Pnpp底物片剂进行检测。
抗地高辛碱性磷酸酶单克隆抗体适用于直接ELISA（1:10000-1:6000）和斑点杂交（以1:20,000稀释，使用地高辛标记的生物分子）。它还用于酶免疫分析（EIA）。
外觀
0.05 M Tris缓冲液，pH 8.0，含1% BSA、50%甘油、1 mM MgCl2和15 mM叠氮化钠
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
J N Mbatha et al.
BMC research notes, 10(1), 702-702 (2017-12-07)
Cervical cancer is a major problem in women and it is important to find a suitable and acceptable screening method, especially among young in low-resource areas for future human papillomavirus (HPV) vaccine follow-up investigations. The study sought to test the
Joyce N Mbatha et al.
Infectious diseases (London, England), 49(8), 601-608 (2017-04-14)
High-risk human papillomavirus (hr-HPV) infections and low-grade squamous intraepithelial lesions occur frequently in young women. The available vaccines cover up to seven hr-HPV genotypes (HPV16, HPV18, HPV31, HPV33, HPV45, HPV52 and HPV58) and two low-risk HPV types (HPV6 and HPV11).
The New England journal of medicine, 336(8), 525-533 (1997-02-20)
The role of cardiac glycosides in treating patients with chronic heart failure and normal sinus rhythm remains controversial. We studied the effect of digoxin on mortality and hospitalization in a randomized, double-blind clinical trial. In the main trial, patients with
Self-sampling for human papillomavirus testing among rural young women of KwaZulu-Natal, South Africa
Mbatha JN, et al.
BMC Research Notes, 10(1), 1-8 (2017)
Liang Wang et al.
Theranostics, 11(2), 925-940 (2021-01-05)
Background: Asparaginase (ASP) is the cornerstone drug in the treatment of extranodal NK/T-cell lymphoma (ENKTCL), and the mechanisms of resistance to ASP remain largely unknown. Long non-coding RNAs play important roles in chemotherapy resistance in various cancers. However, the expression
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